Cell Envelope Morphology of Rumen Bacteria

Cell envelope morphology of rumen bacteria.

The cell walls of three species of rumen bacteria (Bacteroides ruminicola, Bacteroides succinogenes, and Megasphaera elsdenii) were studied by a variety of morphological methods. Although all the cells studied were gram-negative and had typical cytoplasmic membranes and outer membranes, great variation was observed in the thickness of their peptidoglycan layers. Megasphaera elsdenii evidenced a...

Cell Envelope Structure and Functions in Gram-negative Bacteria

Isolation of bacteria envelope proteins.

Proteomic analysis on cell envelope proteins from Gram-negative bacteria requires specific isolation techniques. We found that conventional extraction methods such as osmotic shock cause extracts to be heavily contaminated with soluble cytoplasmic proteins. These cytoplasmic protein contaminants constitute the major signal in proteomic analysis and can overwhelm the signals coming from genuine ...

Lipid Metabolism of Rumen Ciliates and Bacteria

WILLIAMS, P. P. (U.S. Department of Agriculture, Beltsville, Md.), J. GUTIERREZ, AND R. E. DAVIS. Lipid metabolism of rumen ciliates and bacteria. II. Uptake of fatty acids and lipid analysis of Isotricha intestinalis and rumen bacteria with further information on Entodinium simplex. Appl. Microbiol. 11:260-264. 1963.-The total lipid and free fatty acid contents of Isotricha intestinalis, Entod...

Diversity of Rumen Bacteria in Canadian Cervids

Interest in the bacteria responsible for the breakdown of lignocellulosic feedstuffs within the rumen has increased due to their potential utility in industrial applications. To date, most studies have focused on bacteria from domesticated ruminants. We have expanded the knowledge of the microbial ecology of ruminants by examining the bacterial populations found in the rumen of non-domesticated...